Morphological changes of apoptosis and cytotoxic effects induced by Caffeic acid phenethyl ester in AGS human gastric cancer cell line

Nematollah Amini-Sarteshnizi; Saber Zahri; Hamideh Jafari-Ghahfarokhi; Fattane Keivani Hafshejani; Hossein Teimori

J Herbmed Pharmacol. 2014;3(2): 77-82.

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Original Article

Morphological changes of apoptosis and cytotoxic effects induced by Caffeic acid phenethyl ester in AGS human gastric cancer cell line

Nematollah Amini-Sarteshnizi ¹, Saber Zahri ², Hamideh Jafari-Ghahfarokhi ³, Fattane Keivani Hafshejani ³, Hossein Teimori ³^*

¹ MSc Student of Cellular and Molecular Biology, Department of Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Iran
² Department of Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, Iran
³ Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran

*Corresponding Author: *Corresponding author: Hossein Teimori, Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Rahmatiyeh, Shahrekord, Iran. Tel.: +98-381-3346692, Fax: +98-381-3330709, Cell phone: +98-913-3054781. , Email: hteimori@skums.ac.ir

Abstract

Introduction: Gastric cancer is the fourth prevalent cancer and the second reason for cancer-associated mortalities worldwide. Caffeic acid phenethyl ester (CAPE) is one of the main medicinal components of propolis. The aim of this study was to investigate the morphological apoptotic changes and cytotoxic effects of CAPE in human gastric adenocarcinoma cell line (AGS cell).

Methods: AGS human gastric cancer cell line was cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) medium in vitro. Cytotoxic effects and morphological changes induced by 72 h treatment with CAPE at different concentrations on AGS cells were investigated by MTT assay test and inverted microscope, respectively.

Results: CAPE in a concentration dependent fashion reduced viability of AGS cells. IC₅₀ was obtained approximately 10 μM at 72 h treatment. Also, CAPE induced concentration-dependent morphological apoptotic changes and promoted complete apoptosis program in AGS human gastric cancer cell line.

Conclusion: Our results strongly suggest that CAPE stimulates apoptotic process and leads to cell death. Therefore, CAPE could be useful in developing chemotherapeutic agents for treating human gastric cancer.

Keywords: AGS cells, CAPE, Apoptosis, Morphological changes, Cytotoxicity

Implication for health policy/practice/research/medical education:

Caffeic acid phenethyl ester (CAPE) prevents growth and proliferation of AGS cells due to apoptotic process. Therefore, CAPE might be useful in developing chemotherapeutic agents for treating human gastric cancer.

Please cite this paper as: Amini-Sarteshnizi N, Zahri S, Jafari-Ghahfarokhi, Keivani Hafshejani F, Teimori H. Morphological changes of apoptosis and cytotoxic effects induced by Caffeic acid phenethyl ester in AGS human gastric cancer cell line. J Herbmed Pharmacol. 2014; 3(2):77-82.

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