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J Herbmed Pharmacol. 2013;2: 35-40.
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  PDF Download: 6180

Original Article

Biochemical, liver and renal toxicities of Melissa officinals hydroalcoholic extract on balb/C mice

Abdolrasool Namjoo 1, Mohammad MirVakili 2, Hedayatollah Shirzad 3*, Mostafa Faghani 1

1 Pathology Department, Islamic Azad University of Shahrekord Branch, Shahrekord, Iran
2 Veterinary Medicine Department, Islamic Azad University of Shahrekord Branch, Shahrekord, Iran
3 Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
*Corresponding Author: *Corresponding author: Hedayatollah Shirzad, Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran., Email: shirzadeh@yahoo.com

Abstract

Introduction: Melissa officinalis is usually used as antispasmodic, antiaxiety and antibacterial agent. However, its toxicity has not been evaluated, yet. In this study biochemical, liver and renal toxicities of Melissa officinals hydroalcoholic extract were evaluated in balb/C mice.

Methods: In an experimental study, 21 balb/C male mice were randomly designated to three equal groups. Group I was treated with normal saline and groups II and III were respectively treated with 0.450 and 1.350 g/kg, hydroalcoholic extract of Melissa officinals daily for two weeks, intraperitoneally. Then on 15th day of the experiment, blood samples were obtained from the heart. The blood was centrifuged and then the sera were evaluated for alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, urea and creatinine, using autoanalyzer and commercial kits. The liver and kidney tissues were also hystopathologically evaluated. The data were analyzed by one-way ANOVA, Tukey’s post hoc test, and Kruskal-Wallis at a significance level of p<0.05.

Results: Melissa officinals dose dependently caused a significant reduction in alkaline phosphatase and alanine aminotransferase levels compared to the control group. Furthermore, Melissa officinals extract had no effect on the amount of urea and creatinine compared to the control group. The liver and kidney histopathological changes in the groups that received different doses of the extract showed mild, moderate, and severe tissue injuries.

Conclusion: The biochemical analysis in this study indicates that the extract of Melissa officinals causes liver tissue damage in mice; therefore, its consumption in high doses should be avoided.

Keywords: Hepathotoxicity, Melissa officinals, Renotoxicity, Toxicity

Implication for health policy/practice/research/medical education:

Melissa officinalis may cause liver tissue damage and therefore, its consumption in high doses should be avoided.

Please cite this paper as: Namjoo AR, MirVakili M, Shirzad H, Faghani M. Biochemical, liver and renal toxicities of Melissa officinals hydroalcoholic extract on balb/C mice. J Herbmed Pharmacol. 2013; 2(2): 35-40.

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ePublished: 01 Dec 2013
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