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J Herbmed Pharmacol. 2014;3(2): 101-105.
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Original Article

Anti-angiogenesis effect of crocous sativus L. extract on matrix metalloproteinase gene activities in human breast carcinoma cells

Marzieh Mousavi 1, Javad Baharara 2*, Majid Asadi-Samani 3

1 Young Researchers and Elite Club, Mashhad Branch, Islamic Azad University, Mashhad, Iran
2 Research Center for Animal Development Applied Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran
3 Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
*Corresponding author: Javad Baharara, Rahnamaie Ave, Street No. 24. Research Center for Animal Development Applied Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran. Tel & Fax: +985138437092, baharara@mshdiau.ac.ir

Abstract

Introduction: There is an interest in Crocous sativus L. (Saffron) mainly because of its biological properties. Biomedical research has focused on saffron as a powerful antioxidant, anti-inflammatory and anti-tumor, but its mechanism has not yet been thoroughly clarified. In this study, the effects of saffron aqua extract on matrix metalloproteinases (MMP) gene expression were investigated.

Methods: In this experimental study, the saffron was extracted using water as solvent. MCF-7 cells in RPMI1640 medium were supplemented with 10% FBS and incubated at 37 ˚C with 5% CO2. After 24 h, the cells were treated by saffron extract at concentrations of 100, 200, 400 and 800 μg/ml. 48 h after treatment, total RNA was extracted and cDNA was synthesized using specific primer. Synthesized products were analyzed by Real Time PCR to determine expression level of MMP.

Results: Data analysis showed inhibitory effect of saffron at concentrations of 100 to 800 μg/ml on MMP gene expression in comparison with control group. Reduction for 100, 200, 400 and 800 μg/ml were 5%, 18%, 15%, 11%, respectively. According to data analysis treating MCF-7 cells with saffron at concentration of 200 μg/ml caused the highest decrease, with 18% reduction in gene expression (P<0.001).

Conclusion: Results indicate decrease in the expression of MMP compared with controls revealing induction of inhibitory effects of saffron on angiogenesis which might be also considered as a promising chemotherapeutic agent in breast cancer treatment.

Keywords: Crocous Sativus L, Angiogenesis, Matrix metalloproteinase, MCF-7 Cell Line, Cancer

Implication for health policy/practice/research/medical education:

In this study saffron decreased the expression of MMP revealing an inhibitory effect on angiogenesis. This might be considered as a promising chemotherapeutic agent in breast cancer treatment.

Please cite this paper as: Mousavi M, Baharara J, Asadi-Samani M. Anti-angiogenesis effect of crocous sativus L. extract on matrix metalloproteinase gene activities in human breast carcinoma cells. J Herbmed Pharmacol. 2014; 3(2): 101-105.

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ePublished: 01 Dec 2014
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