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J Herbmed Pharmacol. 2014;3(2): 125-128.
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Original Article

Effects of Chrysin plant flavonoid on proliferation and apoptosis of gastric cancer cell line (AGS)

Narges Askarian Dehkordi 1, Hedayatollah Shirzad 2*, Loghman Salimzadeh 3*, Behnam Zamanzad 2, Rezvan Meshkate 1, Mahbube Kiyani 1

1 Department of Immunology, Shahrekord University of Medical Sciences, Shahrekord, Iran
2 Cell and Molecular, Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
3 Medical Plants Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
*Corresponding authors: & Loghman Salimzadeh; Loghman Salimzadeh; Email: Loghman_salim@yahoo.com

Abstract

Introduction: Chrysin is a natural flavonoid antioxidant which its role in tumor cell death has been reported. The aim of this study was to investigate the mechanism of Chrysin effect on proliferation and apoptosis of gastric cancer cell line (AGS).

Methods: Cells were cultured and treated with different concentrations of Chrysin (90, 80, 70, 60, 50 μM) for 48 h and evaluated for cell viability. To examine the cytotoxic effect of drug in inducing apoptosis, staining with fluorescein isothiocyanate (FITC) and propidium iodide (PI) was used. The cells were treated for 48 h with different concentrations of Chrysin (50 of 90 μM) and examined for the morphological changes. The one-way analysis of variance (ANOVA) and the Excel software were used for data analysis.

Results: Different concentrations of Chrysin significantly inhibited the growth and proliferation of AGS cells (p<0.05). The IC50 dose was determined to be 67.5±0.66. Apoptosis induced by 50 and 70 μM of Chrysin was significantly greater than in untreated cells (p<0.05). Cells treated with high concentration of Chrysin (90 μM) showed more prominent growth inhibition and cell shrinkage compared to cells treated with the lower concentrations of Chrysin.

Conclusion: The results of this study showed that Chrysin effects on AGS cell line were significantly high and dose-dependent and might be helpful in the treatment of gastric cancer. Chrysin may therefore be considered a potential candidate for both cancer prevention and treatment. Further investigation is needed to validate the contribution of chrysin in tumor therapy in vivo.

Keywords: Chrysin, Gastric cancer, Apoptosis

Implication for health policy/practice/research/medical education:

Chrysin might be considered as a potential candidate for cancer prevention and treatment. Further investigation is needed to validate the contribution of chrysin in tumor therapy in vivo.

Please cite this paper as: Askarian-Dehkordi N, Shirzad H, Salimzadeh L, Zamanzad B, Meshkate R, Kiyani M. Effects of Chrysin plant flavonoid on proliferation and apoptosis of gastric cancer cell line (AGS). J Herbmed Pharmacol. 2014; 3(2): 125-128.

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ePublished: 01 Dec 2014
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