J Herbmed Pharmacol. 2015;4(1):15-19.
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Original Article

Cytotoxic effect of Cousinia verbascifolia Bunge against OVCAR-3 and HT-29 cancer cells

Seyed Ebrahim Sajjadi 1, Mustafa Ghanadian 2,1 * , Mehrangiz Haghighi 1, Leili Mouhebat 3

1 Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
2 Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
3 Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
*Corresponding author: Mustafa Ghanadian, Department of Pharmacognosy, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran. Tel: +98-3137922553, Email: ghannadian@gmail.com

Abstract

Introduction: Little information is available about phytochemical and biological properties of Cousinia genus. In a primary study, seven Cousinia species including C. verbascifolia showed cytotoxic activity ranged between 18.4 ± 0.59 to 87.9 ± 0.58 μg/mL. To the best of our knowledge, no other biological studies have been conducted on this plant. Therefore, in this study the cytotoxic effect of Cousinia verbascifolia Bunge against OVCAR-3 and HT-29 cancer cells was evaluated.

Methods: Filtration and in vacuo concentration of methanol extract resulted in a green gum which was subjected on reverse column chromatography. Semi polar fraction (41.3 g) eluted with water: methanol (20:80), was then subjected on a silica gel column chromatography using hexane/acetone and resulted in 11 fractions. Finally, cytotoxic activities against ovarian and colon cancer cells were determined at a wavelength of 570 nm by Matrix metalloproteinase protein (MTT) standard method.

Results: None of the fractions showed highly cytotoxic activity. Based on NCI, fractions Fr. 1, Fr. 2, Fr. 4, Fr. 5, Fr. 6, Fr. 8 and Fr. 10 showed moderately cytotoxicity with IC50 values ranged between 119 to 190 μg/mL against OVCAR-3 cells. Fractions Fr. 1, Fr. 2, Fr. 6, Fr. 7 and Fr. 8 showed moderately cytotoxic activity ranged between 118 to 194 μg/mL against HT-29 cells. Fr. 10 and Fr. 11 showed no cytotoxic activity.

Conclusion: Due to the inhibitory properties of extract and its fractions on cancer cells, identification of responsible compounds possessing cytotoxic effects for generating possible new approach in medicinal chemistry are recommended.

Implication for health policy/practice/research/medical education:

In the present study, we evaluated the cytotoxicity properties of Cousinia verbascifolia fractions on cancer cells of ovarian and colon cancer cells. Moderated toxicity of fractions indicated that this plant has the value of more phytochemical consideration to found anti-cancer lead compounds and generating new achievements in medicinal chemistry.

Please cite this paper as: Sajjadi SE, Ghanadian M, Haghighi M, Mouhebat L. Cytotoxic effect of Cousinia verbascifolia Bunge against OVCAR-3 and HT-29 cancer cells . J Herbmed Pharmacol 2015; 4(1): 15-19.

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