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J Herbmed Pharmacol. 2015;4: 29-34.
  Abstract View: 3252
  PDF Download: 1926

Original Article

Anticancer activity of ethanolic extract of propolis on AGS cell line

Nematallah Amini-Sarteshnizi 1, Mohsen Mobini-Dehkordi 2, Somayeh Khosravi-Farsani 3, Hossein Teimori 4*

1 MSc Student of Cellular and Molecular Biology, Department of Biology, Faculty of Science, University of Mohaghegh Ardabili, Ardabil, Iran.
2 Assistant Professor of Molecular Microbiology, Genetics Department, Faculty of Science, University of Shahrekord, Shahrekord, Iran.
3 Assistant Professor of Anatomical Sciences, Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
4 Assistant Professor of Medical Genetics, Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
*Corresponding Author: *Corresponding author: Teimori H (PhD), Cellular and Molecular Research Center, Shahrekord University of Medical Science, Shahrekord, Iran, , Email: hteimori@skums.ac.ir

Abstract

Introduction: Propolis is a natural product derived from various plant resins collected by honeybees, and has been used as a folk medicine for centuries. Propolis has been reported to exhibit a broad spectrum of activities including antibacterial, antifungal, antiviral, anti-inflammatory, antioxidant, hepatoprotective, and anticancer properties. The aim of this study was to investigate the effect of ethanolic extract of propolis (EEP) obtained from Dinaran area (Iran) on AGS human gastric cancer cell line.

Methods: The ethanolic extract of samples was obtained by ethanol 96% and pure extract was dissolved in dimethyl sulfoxide (DMSO) and used for experiments. The cytotoxic effects of various concentrations of EEP on AGS cells were investigated by MTT assay test after 24, 48, and 72 hours and compared with control cells.

Results: The EEP inhibited the growth and proliferation of AGS human gastric cancer cell line. The antiproliferative effects were revealed in a dose and time-dependent manner. The IC50 values were recorded as 60, 30, and 15 (μg/ml) in treatment times of 24, 48 and 72 hours, respectively.

Conclusion: These findings indicated that the native EEP has strong antiproliferative effects against cancerous AGS cells. Thus, propolis and related products may provide a novel approach to the chemoprevention and treatment of human gastric cancer.

Keywords: Propolis, AGSMTT, Viability

Implication for health policy/practice/research/medical education:

Ethanolic extract of propolis prevents growth and proliferation of AGS cells due to strong cytotoxic effects against tumor cells. Therefore, this compound of honey bee might be useful in developing chemotherapeutic agents for treating human gastric cancer.

Please cite this paper as: Amini-Sarteshnizi N, Mobini-Dehkordi M, Khosravi-Farsani S, Teimori H. Anticancer activity of ethanolic extract of propolis on AGS cell line. J Herbmed Pharmacol 2015; 4(1): 29-34.

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