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J Herbmed Pharmacol. 2015;4(2): 65-68.
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  PDF Download: 711

Original Article

Effect of Epigallocatechin-3-gallate (EGCG) on cell proliferation inhibition and apoptosis induction in lymphoblastic leukemia cell line

Masome Ghasemi-Pirbaluti 1, Batool Pourgheysari 2*, Hedayatollah Shirzad 3, Ehsan Motaghi 4, Narges Askarian Dehkordi 1, Zahra Surani 1, Moein Shirzad 5, Pezhman Beshkar 6, Ashkan Pirayesh 1

1 Departmen of Immunology, Shahrekord University of Medical Science, Shahrekord, Iran
2 Medical Plant Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
3 Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
4 Departmen of Pharmacology, Isfahan University of Medical Sciences, Isfahan, Iran
5 Departmen of Clinical Biochemistry, Shahrekord University of Medical Science, Shahrekord, Iran
6 Clinical Biochemistry Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
*Corresponding author: Batool Pourgheysari, Bat238@yahoo.com

Abstract

Introduction: Acute lymphoblastic leukemia (ALL) is one of the malignant proliferations of lymphoid cells in the early stages of differentiation and accounts for ¾ of all cases of childhood leukemia. Available treatment cannot completely treat this disease. Epigallocatechin-3-gallate (EGCG) is a polyphenolic compounds in the green tea that has demonstrated to have anticancer and antimitotic properties. The purpose of the present study was the evaluation of the effect of EGCG on the proliferation inhibition and apoptosis induction in a lymphoblastic leukemia cell line.

Methods: Jurkat cell line was cultured in standard condition and in different concentrations of EGCG (0-100 micromolar) for 24, 48 and 72 hours. Cell viability was measured by MTS assay. Apoptosis induction was assessed by annexin V-FITC and flow cytometry analysis.

Results: The MTS assay revealed that EGCG has decreased cell viability with a time and dose dependent manner. The level of cell apoptosis in all used concentrations of EGCG (50, 70 and 100 μm) was higher than control group (71%, 40% and 31% respectively vs. 8%) and reached to significant level at 100 μm concentration.

Conclusion: The study indicated that EGCG is effective on proliferation inhibition and apoptotic induction in Jurkat lymphoblastic cell line. Therefore, the study of the mechanism of apoptosis induction could be a step of progress toward target therapy which might be considered in the future studies.

Keywords: EGCG, Proliferation, Jurkat cell line, Acute lymphoblastic leukemia, Apoptosis

Implication for health policy/practice/research/medical education:

EGCG has anti-cancer properties against different cell lines and this effect seems to be through apoptosis induction in lymphoblastic leukemia. These results can be a step toward targeted combination or alternative chemotherapy in cancer.

Please cite this paper as: Ghasemi-Pirbaluti M, Pourgheysari B, Shirzad H, Motaghi E, Askarian Dehkordi N, Surani Z, et al. Effect of Epigallocatechin-3-gallate (EGCG) on cell proliferation inhibition and apoptosis induction in lymphoblastic leukemia cell line. J HerbMed Pharmacol 2015; 4(2): 65-68.

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