J Herbmed Pharmacol. 2020;9: 20-27.
doi: 10.15171/jhp.2020.03

Scopus ID: 85078266575
  Abstract View: 1873
  PDF Download: 986

Original Article

Phytoconstituents and bioactivities of the Bark of Pleiogynium timorense (DC.) Leenh (Anacardiaceae)

Gehan Fawzy Abdel Raoof 1* ORCID logo, Ataa Abdelhaleem Said 1, Khaled Younes Mohamed 2, Hesham A. Gomaa 3, 4

1 Pharmacognosy Department, National Research Centre, Dokki, Giza, Egypt
2 Internal Medicine Department, Medical Division, National Research Centre, Giza, Egypt
3 Biochemistry Department, Faculty of pharmacy, Nahda university, Beni-Suef, Egypt
4 Pharmacology Department, College of Pharmacy, Jouf University, Sakakah, Saudi Arabia
*Corresponding Author: *Corresponding author: Gehan Fawzy Abdel Raoof, Email:, Email: ahmedkhaled_1@hotmail.com


Introduction: The purpose of this study was to evaluate the phytoconstituents and various bioactivities of Pleiogynium timorense bark as a step towards the production of a new drug from natural origin to overcome the complications of the synthetic drugs.

Methods: The phenolic compounds were isolated and identified by chromatographic and spectroscopic methods as ultraviolet (UV) and nuclear magnetic resonance (NMR) spectra. The isolated compounds, as well as 70% methanol extract of P. timorense bark were tested for cytotoxicity against human colon carcinoma (HCT 116), human hepatocellular liver carcinoma (HepG2), normal melanocytes (HFB-4) and human breast carcinoma (MCF-7) cell lines. In addition, the methanol extract was evaluated for renal protective, hepatoprotective, antioxidant and antihyperglycaemic activities.

Results: Seven phenolic compounds were isolated from the bark of the plant for the first time which were identified as; pyrogallol, catechin, gallic acid, kaempferol, quercetin, rutin and quercetrin. Moreover, the methanol extract of the bark showed a promising cytotoxic effect against HepG2 cell line more than that of the isolated compounds comparing with doxorubicin (a positive control), where catechin and gallic acid showed moderate effects. In addition, the methanol extract showed potent antioxidant, hepatorenal protective and antihyperglycaemic effects.

Conclusion: Pleiogynium timorense extract possesses a potent cytotoxic effect against HepG2 cell line and significant antioxidant, hepatorenal protective and antihyperglycaemic effects.

Keywords: Antihyperglycaemic agents, Antioxidant, Cytotoxic activity, Hepatorenal toxicity, Phenolic compound, Pleiogynium

Implication for health policy/practice/research/medical education:

The results of this study suggest that the methanol extract of Pleiogynium timorense bark, as well as catechin and gallic acid can be used as promising cytotoxic agents against HepG2 cell line. Moreover, the methanol extract can be used as a potent antioxidant, hepatorenal protective and antihyperglycaemic agent upon further clinical studies.

Please cite this paper as:Abdel Raoof GF, Said AA, Mohamed KY, Gomaa HA. Phytoconstituents and bioactivities of the bark of Pleiogynium timorense (DC.) Leenh (Anacardiaceae). J Herbmed Pharmacol. 2020;9(1):20-27. doi: 10.15171/jhp.2020.03.

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Submitted: 13 Feb 2019
Accepted: 02 Jun 2019
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