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J Herbmed Pharmacol. 2019;8(2): 160-162.
doi: 10.15171/jhp.2019.25

Scopus ID: 85065141272
  Abstract View: 1203
  PDF Download: 810

Short Communication

Genotoxic potential of selected medicinal plant extracts in human whole blood cultures

Karina Harutyunyan 1 ORCID logo, Karine Balayan 2, Gohar Tadevosyan 3, Manush Hayrapetyan 1, Ruzanna Musayelyan 1, Ruzanna Grigoryan 3, Lusine Khondkaryan 3, Natalya Sarkisyan 3, Nelly Babayan 3, 4 * ORCID logo

1 Artsakh Scientific Centre, Stepanakert, RA
2 Department of Biology, Artsakh State University, Stepanakert, RA
3 Group of Cell Technologies, Institute of Molecular Biology, National Academy of Sciences, Yerevan, RA
4 Department of Genetics and Cytology, Yerevan State University, Yerevan, RA

Abstract

Introduction: Many plant-derived products despite wide usage are not scientifically evaluated for their safety and efficacy. Therefore, in the present study, we aimed to evaluate the cytotoxic and genotoxic activities of Polygonum aviculare L., Equisetum arvense L., Plantago lanceolata L. and Artemisia absinthium L. ethanolic extracts in human white blood cells. Methods: Cell viability was assayed by trypan blue exclusion method, while the genotoxicity was tested by cytokinesis-block micronucleus (CBMN) assay upon cells stimulation with noncytotoxic concentrations of the plant extracts. Results: None of the plant extracts showed high cytotoxic activity. At the same time, only extract of P. lanceolata did not present any mutagenic activity, while E. arvense, P. aviculare and A. absinthium were clearly genotoxic. Conclusion: Caution is advice in the case of long-term use of E. arvense, P. aviculare and A. absinthium herbal medicines by population.
Keywords: Medicinal plants, Herbal medicine, Genotoxicity, Cell viability, Micronucleus, Herbal medicine
Introduction: Many plant-derived products despite wide usage are not scientifically evaluated for their safety and efficacy. Therefore, in the present study, we aimed to evaluate the cytotoxic and genotoxic activities of Polygonum aviculare L., Equisetum arvense L., Plantago lanceolata L. and Artemisia absinthium L. ethanolic extracts in human white blood cells. Methods: Cell viability was assayed by trypan blue exclusion method, while the genotoxicity was tested by cytokinesis-block micronucleus (CBMN) assay upon cells stimulation with non-cytotoxic concentrations of the plant extracts. Results: None of the plant extracts showed high cytotoxic activity. At the same time, only extract of P. lanceolata did not present any mutagenic activity, while E. arvense, P. aviculare and A. absinthium were clearly genotoxic. Conclusion: Caution is advice in the case of long-term use of E. arvense, P. aviculare and A. absinthium herbal medicines by population.
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Submitted: 16 Aug 2018
Revision: 08 Nov 2018
Accepted: 16 Nov 2018
ePublished: 25 Feb 2019
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