J Herbmed Pharmacol. 2019;8(3):256-260.
doi: 10.15171/jhp.2019.37
  Abstract View: 32
  PDF Download: 43

Short Communication

In vitro antioxidant effects of different extracts obtained from the leaves and seeds of Allium ampeloprasum subsp. persicum

Saba Feghhi-Najafabadi 1 ORCiD, Leila Safaeian 2 * ORCiD, Behzad Zolfaghari 3

1 Student Research Committee, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
2 Department of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
3 Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Introduction: This study evaluated antioxidant effects of hexane, chloroform, chloroform-methanol, aqueous and butanol extracts of leaves and seeds of Allium ampeloprasum subsp. persicum.Methods: Various extracts were evaluated for total phenolic content, ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH) and H2O2 scavenging activities.Results: Total phenolic content varied from 2.46 to 8.12 mg gallic acid equivalents (GAE) per gram for various extracts with the highest level for butanol leave extract. Butanol leave extract showed the lowest IC50 of DPPH scavenging. FRAP assay showed stronger antioxidant capacity for leaves than seeds’ extracts and butanol extract was comparable to ascorbic acid at the concentration 50 μg/mL. In H2O2 scavenging activity assay, butanol and chloroform-methanol leave extracts showed the least IC50 value.Conclusion: Our results revealed moderately low amount of phenolic compounds and weak DPPH scavenging activity for all extracts of A. ampeloprasum subsp. persicum. However, leaves extracts showed good total antioxidant capacity and H2O2 scavenging activity.
Introduction: This study evaluated antioxidant effects of hexane, chloroform, chloroform-methanol, aqueous and butanol extracts of leaves and seeds of Allium ampeloprasum subsp. persicum.Methods: Various extracts were evaluated for total phenolic content, ferric reducing antioxidant power (FRAP), 1,1-diphenyl-2-picrylhydrazyl (DPPH) and H2O2 scavenging activities.Results: Total phenolic content varied from 2.46 to 8.12 mg gallic acid equivalents (GAE) per gram for various extracts with the highest level for butanol leave extract. Butanol leave extract showed the lowest IC50 of DPPH scavenging. FRAP assay showed stronger antioxidant capacity for leaves than seeds’ extracts and butanol extract was comparable to ascorbic acid at the concentration 50 μg/mL. In H2O2 scavenging activity assay, butanol and chloroform-methanol leave extracts showed the least IC50 value.Conclusion: Our results revealed moderately low amount of phenolic compounds and weak DPPH scavenging activity for all extracts of A. ampeloprasum subsp. persicum. However, leaves extracts showed good total antioxidant capacity and H2O2 scavenging activity.
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Submitted: 10 Mar 2019
Revised: 11 Mar 2019
Accepted: 06 Apr 2019
First published online: 09 May 2019
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