Logo-jhp
J Herbmed Pharmacol. 2022;11: 114-120.
doi: 10.34172/jhp.2022.13
  Abstract View: 310
  PDF Download: 184

Original Article

Preparation of chelidonine highly loaded poly(lactide-co-glycolide)-based nanoparticles using a single emulsion method: Cytotoxic effect on MDA-MB-231 cell line

Zahra Hamidia 1 ORCID logo, Kahin Shahanipour 1* ORCID logo, Nasrin Talebian 2 ORCID logo, Ramesh Monajemi 3 ORCID logo

1 Department of Biochemistry, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
2 Department of Chemistry, Shahreza Branch, Islamic Azad University, Shahreza, Iran
3 Department of Biology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
*Corresponding Author: Email: Shahanipur_k@yahoo.com

Abstract

Introduction: Chelidonine, a bio-active component of Chelidonium majus, has been investigated for its anti-proliferative effects on various cancer cell lines with multidrug resistance (MDR). Although the results are auspicious, its poor water solubility and low bioavailability are the main limitations for clinical applications. This study aimed to develop poly(lactic-co-glycolic acid) (PLGA) nanoparticles loaded with chelidonine, in order to enhance its bioavailability for oral administration and improve the therapeutic index. Methods: Herein, we encapsulated chelidonine in PLGA nanoparticles using a single emulsion solvent evaporation method. Nanoparticles were characterized in terms of size, surface charge and morphology, encapsulation efficiency, drug loading, and in vitro drug release profile. The anti-cancer efficacy of chelidonine-loaded nanoparticles and free chelidonine was evaluated in MDA-MB-231 breast cancer cells. Results: The physicochemical characteristics showed spherical particles in nanometer size range (263 ± 19.6 nm), with negative surface charge (−20.67 ± 2.48 mv), high encapsulation efficiency (76.53 ± 3.61%), and drug loading (22.47 ± 0.09%), as well as drug release amount of 60.27±5.68% up to 10 days. Furthermore, chelidonine-loaded nanoformulations were found to improve anti-cancer potential, compared with unentrapped chelidonine. Conclusion: This in vitro study showed that the encapsulation of chelidonine, as a potent herbal drug, in a polymeric matrix enhances its bioavailability. This offers an efficient vehicle for targeted drug delivery in cancer treatment.
Keywords: Chelidonium majus, Bioavailability, Nanoparticles, Drug delivery, Herbal Drug, Breast Cancer

This study revealed that encapsulation of chelidonine in PLGA nanoparticles led to a higher cytotoxic effect on MDA-MB-231 cancer cells, in comparison with free chelidonine, which offers a potential therapeutic approach for cancer treatment in the future.
First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 311

Your browser does not support the canvas element.


PDF Download: 184

Your browser does not support the canvas element.

Submitted: 10 Jun 2021
Revision: 26 Oct 2021
Accepted: 27 Oct 2021
ePublished: 29 Nov 2021
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)