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J Herbmed Pharmacol. 2021;10(4): 408-414.
doi: 10.34172/jhp.2021.48
  Abstract View: 274
  PDF Download: 209

Original Article

In vitro inhibitory potentials of ethanolic extract of Moringa oleifera flower against enzymes activities linked to diabetes

Oluwaseun Ruth Olasehinde 1* ORCID logo, Olakunle Bamikole Afolabi 1 ORCID logo, Benjamin Olusola Omiyale 1 ORCID logo, Oyindamola Adeniyi Olaoye 1 ORCID logo

1 College of Science, Department of Chemical Sciences, Biochemistry Programme, Afe Babalola University, P.M.B 5454 Ado-Ekiti, Ekiti State, Nigeria

Abstract

Introduction: Diabetes mellitus (DM) has been recognized as the seventh leading cause of global mortality; however, researchers seek alternative means to manage the menace. The current study sought to investigate antioxidant potentials, α-amylase, and α-glucosidase inhibitory activities of ethanolic extract of Moringa oleifera flower in vitro. Methods: Antioxidant properties of the extract were appraised by assessing its inhibition against 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydroxyl (OH•), and hydrogen peroxide (H2O2) free radicals, as well as ferric reducing antioxidant power (FRAP), the antidiabetic activity was evaluated by α-amylase and α-glucosidase inhibition.Results: In this study, ethanolic extract of M. oleifera flower demonstrated a significant (P < 0.05) inhibition against DPPH free radical (43.57–83.56%) in a concentration-dependent manner, while FRAP (101.76 ± 1.63 mg/100 g), OH• scavenging ability (71.62 ± 0.95 mg/100 g), and H2O2 free radical scavenging capacity (15.33 ± 1.20 mg/100 g) were also observed. In the same manner, ethanolic extract of M. oleifera flower revealed a significant (P < 0.05) inhibition against α-amylase (IC50= 37.63 mg/mL) and α-glucosidase activities (IC50= 38.30 mg/mL) in the presence of their respective substrates in a concentration-dependent manner in comparison with acarbose. Conclusion: Ethanoic extract of M. oleifera flower could be useful as an alternative phytotherapy in the management of DM, having shown a strong antioxidative capacity and substantial inhibition against the activities of key enzymes involved in carbohydrate hydrolysis in vitro.
Keywords: Moringa oleifera, Antioxidant activity, α-Amylase, α-Glucosidase, Type II diabetes

Implication for health policy/practice/research/medical education:

Ethanolic extract of Moringa oleifera flower possesses significant antioxidant and antidiabetic potentials and might be used as an alternative phytotherapy in the management of diabetes mellitus.

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Submitted: 14 Nov 2020
Revision: 28 Apr 2021
Accepted: 29 Apr 2021
ePublished: 29 Sep 2021
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