J Herbmed Pharmacol. 2022;11: 131-139.
doi: 10.34172/jhp.2022.15
  Abstract View: 344
  PDF Download: 173

Original Article

In vitro antidiabetic, antioxidant activities and chemical composition of Ajuga parviflora Benth. shoot

Amrita Suryavanshi 1 ORCID logo, Suresh Kumar 1* ORCID logo, Dolly Kain 1 ORCID logo, Atul Arya 1 ORCID logo, Vandana . 2 ORCID logo

1 Medicinal Plants Research Laboratory, Department of Botany, Ramjas College, University of Delhi, Delhi-110007, India
2 Department of Chemistry, Dyal Singh College, University of Delhi, Delhi-110007, India
*Corresponding Author: Email: suresh.kumar@ramjas.du.ac.in


Introduction: Ajuga parviflora Benth. (Lamiaceae) is an herbaceous plant that possesses ethnomedicinal values and is well known for its folkloric management of diabetes. This study was aimed to provide an experimental justification for its traditional antidiabetic use. Methods: Hydroalcoholic extract of A. parviflora shoot was quantified for its total phenolic content (TPC), total flavonoid content (TFC), and total tannin content (TTC). Gas chromatography-mass spectrometry (GC-MS) and Fourier transform infrared spectrophotometer (FTIR) spectroscopy were also used for their chemical nature. Additionally, the extract was evaluated for its inhibitory potential against key enzymes linked with hyperglycemia by in vitro means. Subsequently, for estimation of the antioxidant capacities 2,2-diphenyl-2-picrylhydrazyl radical (DPPH), 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical (ABTS), and hydrogen peroxide (H2O2) scavenging activities were determined.Results: GC-MS analysis revealed numerous biologically active phytoconstituents including brassicasterol, phytol, and palmitic acid. The presence of different active functional groups such as alcohol, nitrile, amine, alkyl halide, alkene, and alkane was confirmed by FTIR analysis. The extract showed a significant (P≤ 0.05) dose-dependent inhibition for α-amylase enzyme (132.38±1.18 μg/mL), α-glucosidase enzyme (22.66±0.11 μg/mL), DPPH radical (103.03±1.59 μg/mL), ABTS radical (140.10±3.40 μg/mL) and H2O2 radical (298.26±4.37 μg/mL). TPC, TFC, and TTC were found 64.06±0.35 mg/g of the gallic acid equivalent (GAE), 45.27±0.58 mg/g of the rutin equivalent (RE), and 127.42±1.82 mg/g of the tannic acid equivalent (TAE), respectively. Conclusion: A. parviflora extract showed significant antioxidant and antidiabetic potentials. Thus, this plant might be served as a novel approach for discovering new and effective drug molecules against hyperglycemia.
Keywords: Medicinal plant, Functional groups, Bioactive compounds, Radical scavenging activities, α-Amylase, α-Glucosidase

The shoot extract of Ajuga parviflora Benth. possesses antidiabetic and antioxidant properties; therefore, it might be a potential source in the pharmacological industries after searching and isolating effective phyto-molecules against diabetes mellitus.
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Submitted: 19 Apr 2021
Revision: 26 Aug 2021
Accepted: 27 Aug 2021
ePublished: 29 Nov 2021
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