Logo-jhp
J Herbmed Pharmacol. 2021;10(4): 436-442.
doi: 10.34172/jhp.2021.51
  Abstract View: 151
  PDF Download: 127

Original Article

Mechanisms of hepatoprotective effects of white and black garlic extracts against preneoplastic lesions induced by N-nitrosodiethylamine in rats

Mostafa Abbas Shalaby 1* ORCID logo, Rehab Ali Ghandour 2 ORCID logo, Shimaa Ramadan Imam 1 ORCID logo

1 Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, P.O. Box 12211, Egypt
2 Department of Physiology, Faculty of Veterinary Medicine, Cairo University, Giza, P.O. Box 12211, Egypt

Abstract

Introduction: Garlic has many pharmacological properties such as antibacterial, anti-inflammatory, and antioxidant activities. This study aimed to investigate the possible mechanisms of the hepatoprotective effect of white garlic extract (WGE) and black garlic extract (BGE) against preneoplastic lesions induced by N-nitrosodiethylamine (NDEA) in rats. Methods: Forty-two rats were randomly distributed into six equal groups. Oral administration of WGE and BGE began 3 weeks before injection of NDEA. Group 1 was kept as the negative control, while the other groups were injected by a single intraperitoneal dose of NDEA in the 3rd week, followed by 2 subcutaneous injections/week of CCl4 till six weeks to induce preneoplastic lesions. Group 2 kept positive control and groups 3, 4, 5, and 6 were given WGE and BGE each of them at 250 and 500 mg/kg, respectively, for six weeks from the beginning. Serum liver enzymes, total protein, albumin (Alb), total bilirubin (TBil), and antioxidant enzymes were measured. Malondialdehyde (MDA), glutathione (GSH), tumor biomarkers, along with the content of 8-hydroxy-2-deoxyguanosine (8-OHdG) in liver DNA were estimated and liver histopathology was performed. Results: WGE and BGE significantly decreased the serum liver enzymes, TBil, tumor biomarkers, lipid peroxidation but increased total protein levels. The extracts significantly increased antioxidant enzymes, decreased 8-OHdG content in liver DNA, and alleviated histopathological lesions in the liver. Conclusion: The results affirm the hepatoprotective effect of WGE and BGE against NDEA-induced preneoplastic lesions in rats. This effect may be due to inhibition of lipid peroxidation, reduction of tumor biomarkers, enhancement of antioxidant enzymes, or reduction of 8-OHdG content in liver DNA.
Keywords: Garlic, N-nitrosodiethylamine, Serum analysis, Antioxidant, Tumor biomarkers, Histopathology

Implication for health policy/practice/research/medical education:

White and black garlic extracts produce a good protective effect against N-nitrosodiethylamine-induced preneoplastic lesions in rats. This effect might be due to inhibition of lipid peroxidation, increased activity of antioxidant enzymes or reduction of 8-OHdG content in liver DNA. Therefore, they might be useful in these subjects.

First Name
 
Last Name
 
Email Address
 
Comments
 
Security code


Abstract View: 151

Your browser does not support the canvas element.


PDF Download: 127

Your browser does not support the canvas element.

Submitted: 05 May 2021
Revision: 23 Jun 2021
Accepted: 23 Jul 2021
ePublished: 29 Sep 2021
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)