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J Herbmed Pharmacol. 2022;11: 91-98.
doi: 10.34172/jhp.2022.10
  Abstract View: 360
  PDF Download: 207

Original Article

Rhinacanthin-C enhances chemosensitivity of breast cancer cells via the downregulation of P-glycoprotein through inhibition of Akt/NF-kappa B signaling pathway

Suwichak Chaisit 1 ORCID logo, Suree Jianmongkol 2* ORCID logo

1 Inter-Department Program of Pharmacology, Graduate School, Chulalongkorn University, Bangkok 10330, Thailand
2 Department of Pharmacology and Physiology, Faculty of Pharmaceutical Science, Chulalongkorn University, Bangkok 10330, Thailand
*Corresponding Author: Email: suree.j@pharm.chula.ac.th

Abstract

Introduction: High expression of P-glycoprotein (P-gp) has been linked to multidrug resistance (MDR) and chemotherapeutic failure. Previously, we demonstrated that rhinacanthin-C, a naphthoquinone from Rhinacanthus nasutus, was able to enhance the cytotoxicity of doxorubicin against breast cancer MCF-7 cells via direct P-gp inhibition. In this study, we looked at its effect on P-gp downregulation and the mechanism involved in the resistance of MCF-7 cells to doxorubicin. Methods: Doxorubicin-resistant MCF-7 (MCF-7/DOX) cells were exposed to rhinacanthin-C for 24-48 hours prior to the assessment of their chemosensitivity via MTT assay, P-gp activity via calcein-AM uptake assay, P-gp expression, and signaling via qRT-PCR and western blot analyses. Results: Pretreatment with 1 µM of rhinacanthin-C for 48 hours significantly enhanced cytotoxicity of doxorubicin, as well as camptothecin and etoposide, to MCF-7/DOX cells. In the rhinacanthin-C-treated cells, reduction of MDR1 mRNA and P-gp levels and increased intracellular calcein were observed. Moreover, phosphorylation of Akt, NF-ᴋB and IκB-α, along with YB-1 expression, significantly decreased after 24-hour treatment with rhinacanthin-C. In contrast, the naphthoquinone had no effect on expression levels of ERK1/2 and phosphorylated ERK1/2 under similar conditions. Conclusion: Rhinacanthin-C, at a non-cytotoxic concentration (1 µM), could downregulate P-gp expression in MCF-7/DOX cells via the inhibition of the Akt/NF-ᴋB signaling pathway and YB-1 expression. Long-term exposure to this natural naphthoquinone may increase the chemosensitivity of cancer cells with MDR phenotype.
Keywords: Naphthoquinones, ATP binding cassette transporter, Multi-drug resistance, Breast cancer cells, Signal transduction

The present study revealed the suppressive activity of rhinacanthiin-C on P-gp overexpression, which suggested its potential use as a chemosensitizer in cancer therapy.
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Submitted: 08 Jun 2021
Revision: 25 Jul 2021
Accepted: 22 Aug 2021
ePublished: 29 Nov 2021
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