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J Herbmed Pharmacol. 2022;11: 48-54.
doi: 10.34172/jhp.2022.05
  Abstract View: 346
  PDF Download: 290

Original Article

In vitro anti-melanogenic effect of Perilla frutescens leaf extracts

Thi-Thuy-Anh Nguyen 1 ORCID logo, Thanh-Hao Do 2 ORCID logo, Thi-Thu-Hien Pham 3 ORCID logo, Ngoc-Trinh Huynh 1* ORCID logo

1 University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam
2 Hong Bang International University; Ho Chi Minh City, Vietnam
3 International University - Vietnam National University of Ho Chi Minh City, Vietnam
*Corresponding Author: Email: hntrinh@ump.edu.vn

Abstract

Introduction: Perilla frutescens (L.) Britt leaves contain various phenolic compounds, especially flavonoids, which potentially inhibit tyrosinase, the key enzyme catalyzing the melanin synthesis pathway. This work aimed to investigate the anti-melanogenic effect of Perilla frutescens extracts through the inhibition of non-cellular as well as cellular tyrosinase activities. Methods: The total extract from Perilla frutescens leaves was obtained by percolation method with 50% ethanol followed by liquid–liquid partition with ethyl acetate (EA) and chloroform (CF) to obtain the EA, CF and EA/CF extracts. The mushroom tyrosinase inhibitory effect of the obtained extracts was screened by dopachrome formation reactions from its substrate 3,4-dihydroxy-L-phenylalanine. The extracts with potential activity were further evaluated for the anti-melanogenic effects in the B16F10 melanoma cell line. Results: EA and EA/CF extracts significantly inhibited mushroom tyrosinase activity with the IC50s of 0.14 and 0.07 mg/mL, respectively, in the same range of that from kojic acid (0.12 mg/mL). Consistently, in B16F10 cells, these extracts inhibited tyrosinase enzyme; their IC50 values were 2 times lower than that of kojic acid. Moreover, both EA and EA/CF extracts remarkably reduced melanin levels in a concentration-dependent manner. Conclusion: The EA and EA/CF extracts from Perilla frutescens leaves were able to inhibit melanogenesis by reducing tyrosinase activity.
Keywords: B16F10 cell line, Perilla frutescens, Melanin, Tyrosinase activity

The results of this study showed the promising anti-melanogenic effect of various extracts from Perilla frutescens leaves providing the scientific evidence for the topical application of Perilla for skin whitening or pigmentation prevention.
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Submitted: 01 Jul 2021
Revision: 24 Aug 2021
Accepted: 26 Aug 2021
ePublished: 29 Nov 2021
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