J Herbmed Pharmacol. 2023;12: 505-511.
doi: 10.34172/jhp.2023.45000
  Abstract View: 317
  PDF Download: 123

Original Article

Phytochemical and anticandidal efficacy of Olea europaea leaf extract from different cultivars and seasonal variation

Pedram Alimosazadeh 1 ORCID logo, Mohd Sajjad Ahmad Khan 2 ORCID logo, Seyed Javad Sanei 1* ORCID logo

1 Department of Plant Protection, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
2 Department of Biology, College of Medicine, Imam Abdulrahman Bin Faisal University, Dammam 31451, Saudi Arabia
*Corresponding Author: Seyed Javad Sanei, Email: sa_nei@gau.ac.ir


Introduction: Olive (Olea europaea L.) is one of the important agricultural and medicinal tree species. This study aimed to assess the antifungal efficacy of olive leaf extract (OLE) obtained from different cultivars and during different seasons against Candida albicans strains.

Methods: OLE was prepared using four olive cultivars (‘Koroneiki’, ‘Mission’, ‘Rowghani’, and ‘Zard’) obtained in two seasons (spring and autumn) from Golestan province, North of Iran. The phenolic and oleuropein contents of vegetative leaves were measured by colorimetric and HPLC techniques, respectively. The antifungal capacities of OLEs were tested by agar well diffusion and the minimal inhibitory concentration (MIC) was evaluated by micro-dilution assay.

Results: The findings of our study showed that the total phenolic (27.45-88.16 mg g-1) and oleuropein (3.64-18.13 mg g-1) contents varied in leaf extracts, respectively. The highest amount was found in ‘Koroneiki’ and ‘Zard’ (spring leaves) and the lowest in ‘Mission’ cultivars (autumn leaves). The inhibition zones and MIC ranged from 1.92 to 15.41 mm and from 6.07 to 27.20 mg mL-1 based on C. albicans strains, respectively. Relationship between total phenolic content as an independent variable (X) and inhibition zones or MIC as dependent variables (Y) fitted polynomial curves.

Conclusion: The present study highlighted the phytochemical and anti-candidal efficacy of OLE derived from olive cultivars or the seasons of harvested leaves against C. albicans strains. It is suggested that ‘Koroneiki’ and ‘Zard’ cultivars, especially during the spring season, could be exploited to isolate potential broad-spectrum antifungal drugs.

Keywords: Antioxidants, Antifungal agent, Candida albicans, Oleuropein, Olive leaf extract

Implication for health policy/practice/research/medical education:

Our findings showed the significant variations of phytochemical profiles and anti-candidal capacities of olive leaves by harvest time and type of cultivar, and spring leaves were suggested as the best raw materials for pharmaceutical industries. The results also emphasized the broad antifungal sensitivity pattern of Candida albicans strains to olive leaf extracts.

Please cite this paper as: Alimosazadeh P, Ahmad Khan MS, Sanei SJ. Phytochemical and anticandidal efficacy of Olea europaea leaf extract from different cultivars and seasonal variation. J Herbmed Pharmacol. 2023;12(4):505-511. doi: 10.34172/ jhp.2023.45000.

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Submitted: 09 Apr 2023
Revision: 09 Jul 2023
Accepted: 09 Jul 2023
ePublished: 10 Aug 2023
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