Shahrekord University of Medical Sciences Journal of Herbmed Pharmacology 2345-5004 2 1 2013 01 01 Reversal effect of Achillea millefolium extract on ileum contractions 5 8 EN Mehrnoosh Sedighi Hamid Nasri Mahmoud Rafieian-kopaei Seifollah Mortazaei Journal Article Introduction: Traditionally Achillea millefolium L. has been used to treat gastrointestinal disorders. In this study the hydroalcoholic extract of A. millefolium was evaluated on ileum contractions of Wistar rats. Materials and Methods: In this experimental study 32 male Wistar rats were designated into 4 equal groups, including: acetyl choline, KCl, extract plus acetyl choline and the group which received extract plus KCl. The isotonic contractions of ileum (induced by 60 mM KCl or 1 μM acetyl choline) in tyrode solution were recorded, under 1 gr tension. Then, the effects of normal saline or extract (1%) were evaluated. The percentage changes were calculated and compared in different groups using ANOWA and Tukey tests. Results: The mean of ileum contractions in acetyl choline and KCl groups were 18.83±4.91 and 18.31±11.12 (p=0.5). The percentage of contraction relieve in extract plus acetyl choline group was 53.16±12.06 and in extract plus KCl group was 62.96±11.08 (p=0.4). The contractions in extract groups were significantly less than acetyl choline or KCl groups (p<0.05). Conclusion: The results indicate that Achillea millefolium extract inhibits ileum contractions. Therefore, it might be used in patients to reduce ileum spasms.