Shahrekord University of Medical Sciences Journal of Herbmed Pharmacology 2345-5004 3 1 2014 01 01 Screening of antibacterial effect of the Scrophularia Striata against E. coli in vitro 31 34 EN Reza Sharafati-chaleshtori Mahmoud Rafieian-kopaei Journal Article Introduction: This study was aimed to evaluate the antimicrobial activity of the ethanol and aqueous extracts of Scrophularia striata plant on E. coli O157:H7 in vitro. Methods: In this experimental study the ethanol and aqueous extract of the plant was prepared and their antibacterial effects were determined using sink diffusion and broth macrodilution methods against the bacterium E. coli O157:H7. Results: The ethanol extract of Scrophularia striata plant had inhibitory effect on the E. coli O157:H7 in two methods of sink diffusion and macrodilution, but the aqueous extract of this plant had not antibacterial effect. The MIC and MBC amounts were obtained 90mg/ml and 100 mg/ml, respectively. Conclusion: Based on the present results that the ethanol extract of the Scrophularia Striata plant showed inhibitory effect on bacterium, more researches are recommended to evaluate its in vivo effects and to identify active compounds.