Shahrekord University of Medical Sciences Journal of Herbmed Pharmacology 2345-5004 15 1 2026 01 01 Investigation of cardioprotective effects of Leea rubra Blume leaves on isoproterenol-induced left ventricular hypertrophy in mice 27 35 10.34172/jhp.2026.53125 EN Md. Abdur Nur Tushar https://orcid.org/0009-0008-7697-4051 Md. Mamunur Rashid https://orcid.org/0009-0001-6816-4752 Farhad Hossain https://orcid.org/0000-0003-1333-474X Kazi Mazharul Islam https://orcid.org/0009-0000-9831-6342 Md. Ariful Islam https://orcid.org/0000-0003-2796-4224 Farjana Sharmin https://orcid.org/0009-0003-7629-2898 Purnima Rani Das https://orcid.org/0009-0006-2080-6417 Mohammad A. Rashid https://orcid.org/0000-0003-1464-7115 AHM Khurshid Alam https://orcid.org/0000-0003-2690-6559 Journal Article 10.34172/jhp.2026.53125 2025 05 01 2025 08 22 Introduction: Cardiac hypertrophy is a risk factor for many cardiovascular diseases (CVDs). Species of the genus Leea have traditional cardioprotective uses, but their direct effects on the heart remain unreported. This study evaluated the cardioprotective activities of a crude methanolic extract (CME) of L. rubra leaves (LRL) in isoproterenol-induced cardiac hypertrophic mice (IIHM) using atorvastatin as a reference treatment. Methods: IIHM (induced with isoproterenol, 5 mg/kg, 7 days, intraperitoneally) were treated with CME of LRL (40 mg/kg or 80 mg/kg, 28 days, orally) and compared with a standard treatment group (atorvastatin, 20 mg/kg, 28 days, orally) and a negative control group (IIHM, placebo). The cardioprotective and hepatoprotective effects of the extract were evaluated by analysing the changes in blood biochemical parameters, including lipid profile (total cholesterol [TC], triglyceride [TG], high-density lipoprotein [HDL], and low-density lipoprotein [LDL]), cardiac troponin I (CTnI), serum glutamic pyruvic transaminase (SGPT), and serum glutamic oxaloacetic transaminase (SGOT). Analysis was conducted using various commercial test kits. The left ventricular weight and body weight (LVW/BW) ratio was also recorded. Results: LRL significantly improved the lipid profile by lowering TC, TG, and LDL while increasing HDL levels (P<0.0001). Significant cardioprotective (reduced serum CTnI level and LVW/BW ratio (P<0.0001)) and hepatoprotective (reduced serum SGPT and SGOT levels (P<0.0001)) activities were observed in IIHM compared with the negative control group. Conclusion: The present study found antihyperlipidemic, cardioprotective, and hepatoprotective activity after LRL treatment in IIHM. Further investigation is required to identify the compounds and molecular mechanisms responsible for these effects.