J Herbmed Pharmacol. 2015;4(4): 110-114.
  Abstract View: 3294
  PDF Download: 2142

Original Article

Effect of Crocus sativus Stigma (saffron) alone or in combination with chloroquine on chloroquine sensitive strain of Plasmodium berghei in mice

Nader Pestechian 1, Somayeh Abedi-Madiseh 1, Mustafa Ghanadian 2, 3*, Mehdi Nateghpour 4

1 Department of Medical Mycoparasitology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
3 Pharmacognosy Department, Isfahan Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran
4 Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
*Corresponding Author: Email: Ghanadian1@skums.ac.ir


Introduction: In malaria treatment protocols, treatment failure or drug resistance of synthesized drugs like alkaloids related to quinine, and aminoquinolines are the main problems now. Therefore, discovering efficient drugs or combination therapy of blood schizonticidal drugs with different mechanisms or different targets in the parasite is a crucial effort to solve this problem. In this study, the effectiveness of Crocus sativus Stigma (saffron) individually and in combination with chloroquine, was considered against chloroquine–sensitive strain of Plasmodium berghei.Methods: At the first stage, using 4 day suppressive Peter’s test in mice, ED50 and survival times of saffron methanol extract, and its aqueous and ethyl acetate fractions and chloroquine on P. berghei were calculated. Then, based on the toxicity and survival time results, combination therapy was conducted with the best saffron fraction and chloroquine against the parasite.Results: The saffron extract, aqueous and ethyl acetate fractions resulted in suppression of parasitemia with ED50 values of 587.0 ± 78.7, 323.7 ± 37.2, and 508.7 ± 35.6 mg/kg, respectively. Combination of ethyl acetate fraction with chloroquine, potentiated the antimalarial property and the survived percent of the treated mice on days 7, 14, and 28 significantly more than chloroquine or ethyl acetate fraction alone.Conclusion: Saffron and its fractions individually can be effective in reducing the parasitemia in mice. The outcome of combination of ethyl acetate fraction with chloroquine on the mice showed synergistic effect on the chloroquine–sensitive strain of parasite.
First Name
Last Name
Email Address
Security code

Abstract View: 3295

Your browser does not support the canvas element.

PDF Download: 2142

Your browser does not support the canvas element.

ePublished: 14 Oct 2015
EndNote EndNote

(Enw Format - Win & Mac)

BibTeX BibTeX

(Bib Format - Win & Mac)

Bookends Bookends

(Ris Format - Mac only)

EasyBib EasyBib

(Ris Format - Win & Mac)

Medlars Medlars

(Txt Format - Win & Mac)

Mendeley Web Mendeley Web
Mendeley Mendeley

(Ris Format - Win & Mac)

Papers Papers

(Ris Format - Win & Mac)

ProCite ProCite

(Ris Format - Win & Mac)

Reference Manager Reference Manager

(Ris Format - Win only)

Refworks Refworks

(Refworks Format - Win & Mac)

Zotero Zotero

(Ris Format - Firefox Plugin)