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J Herbmed Pharmacol. 2023;12(2): 283-298.
doi: 10.34172/jhp.2023.31

Scopus ID: 85158137373
  Abstract View: 1149
  PDF Download: 673

Original Article

In vitro and in silico investigation of garlic’s (Allium sativum) bioactivity against 15-lipoxygenase mediated inflammopathies

Arman Mahmud Khan 1 ORCID logo, Md. Arman Sharif 1 ORCID logo, Rahagir Salekeen 1 ORCID logo, Md. Hafijur Rahman 1 ORCID logo, Sakib Mahmud 1 ORCID logo, Partha Biswas 2 ORCID logo, Md. Nazmul Hasan 2 ORCID logo, Kazi Mohammed Didarul Islam 1 ORCID logo, Md. Morsaline Billah 1 ORCID logo, Md. Emdadul Islam 1* ORCID logo

1 Biotechnology and Genetic Engineering Discipline, Life Science School, Khulna University, Khulna-9208, Bangladesh
2 Department of Genetic Engineering and Biotechnology, Jashore University of Science and Technology, Jashore-7408, Bangladesh
*Corresponding Author: Md. Emdadul Islam, Email: emdad@bge.ku.ac.bd

Abstract

Introduction: Garlic (Allium sativum) is widely used as a flavor-enhancing dietary ingredient and exhibits a wide spectrum of pharmacological effects. This study aimed to investigate the therapeutic effects of aqueous garlic extract to explore the bioactivity against 15-lipoxygenase (15-LOX) mediated inflammopathies.

Methods: In this study, the antioxidant (DPPH free radical scavenging assay and reducing power assay), anti-inflammatory (hypotonicity-induced hemolysis assay and 15-LOX inhibition assay) and anticoagulation (serine protease inhibition assay and prothrombin time assay) effects of the aqueous garlic extract were investigated. Furthermore, in silico molecular docking and dynamic simulation analysis of reported small compounds of garlic against 15- LOX1 and 15-LOX2 were performed to figure out the most efficient phytochemical ligands and validate the anti-inflammatory potential.

Results: The DPPH scavenging effect and the reducing power of the extract were found with the IC50 of 213.87±1.49 µgmL-1 and EC50 of 124.78±3.39 µgmL-1, respectively. In the hypotonicity-induced hemolysis and 15-LOX inhibition assay, the IC50 values were observed as 147.59±2.98 µgmL-1 and 250.05±8.48 µgmL-1, respectively. The extract inhibited serine protease activity with an IC50 of 301.33±1.31 µgmL-1 and prevented blood coagulation for 10.05±0.35 minutes in prothrombin time assay. The in silico study identified Rhamnetin as a potential 15-LOX1 and 15-LOX2 inhibitor, and it exhibited a stable interaction with the targets throughout the 100 ns dynamic simulation.

Conclusion: The findings of this study provide molecular insights into garlic’s medicinal properties as well as its bioactive compounds, which can be potential therapeutic interventions for 15-LOX mediated inflammations.


Implication for health policy/practice/research/medical education:

The findings of this study suggest that bioactive compounds of garlic extract have potent therapeutic features as anti-inflammatory agents that can help to explore and investigate new potentially active therapeutics from garlic against lipoxygenase mediated chronic inflammatory illness.

Please cite this paper as: Khan AM, Sharif MA, Salekeen R, Rahman MH, Mahmud S, Biswas P, et al. In vitro and in silico investigation of garlic’s (Allium sativum) bioactivity against 15-lipoxygenase mediated inflammopathies. J Herbmed Pharmacol. 2023;12(2):283-298. doi: 10.34172/jhp.2023.31.

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Submitted: 30 Aug 2022
Revision: 16 Oct 2022
Accepted: 17 Oct 2022
ePublished: 18 Mar 2023
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