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J Herbmed Pharmacol. 2023;12(1): 147-152.
doi: 10.34172/jhp.2023.14

Scopus ID: 85147750590
  Abstract View: 716
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Original Article

The protective effect of Boesenbergia rotunda extract on cisplatin-exposed human embryonic kidney-293 cells by inhibiting the expression of kidney injury molecule-1, neutrophil gelatinase associated-lipocalin, NF-κB, and caspases

Dani Sujana 1,2 ORCID logo, Nyi Mekar Saptarini 3 ORCID logo, Sri Adi Sumiwi 1 ORCID logo, Jutti Levita 1* ORCID logo

1 Department of Pharmacology and Clinical Pharmacy, Padjadjaran University, Sumedang, West Java, Indonesia 45363
2 Diploma Program of Pharmacy, Karsa Husada Garut College of Health Sciences (STIKes Karsa Husada Garut), Garut, West Java, Indonesia 44151
3 Department of Pharmaceutical Analysis and Medicinal Chemistry, Padjadjaran University, Sumedang, West Java, Indonesia 45363
*Corresponding Author: Jutti Levita, Email: jutti.levita@unpad.ac.id

Abstract

Introduction: Acute kidney injury (AKI) is a major problem in platinum-based chemotherapy patients. Boesenbergia rotunda can induce the generation of osteoblast cells and significantly increase pancreatic antioxidant enzyme activities; therefore, this study aimed to investigate the cytotoxicity of cisplatin on human embryonic kidney-293 (HEK-293) cells and the protective impact of the ethanol extract of B. rotunda (EEBR) against such conditions.

Methods: Cytotoxicity was assessed using the CCK-8/WST-8 reagent, while the protective activity was assayed on 1 µg/mL cisplatin-exposed HEK-293 cells by quantifying the expression of nephrotoxicity biomarkers, e.g., kidney injury molecule-1 (Kim-1) and neutrophil gelatinase associated-lipocalin (NGAL), nuclear factor-kappaB (NF-κB), apoptotic caspase-3, and caspase-7 genes, in cisplatin-exposed HEK-293 cells.

Results: Cisplatin was confirmed as highly toxic against the HEK-293 cells (IC50 = 2.5145 μg/ mL), whereas quercetin was of moderate toxicity (IC50 = 185.6225 μg/mL). EEBR revealed an IC50 = 40.0655 μg/mL. Moreover, EEBR concentrations of 5, 10, and 20 µg/mL confirmed its remarkable protective activity against cisplatin-exposed HEK-293 cells (P=0.031, 0.014, 0.046, respectively) compared to the cisplatin-treated cell lines without treatment. The quantitative real-time polymerase chain reaction (PCR) revealed that a higher concentration of EEBR significantly suppressed the expression of Kim-1, while lower concentrations of EEBR significantly inhibited NGAL and NF-κB genes. Higher concentrations of EEBR reduced the expression of caspase-3. All concentrations of EEBR stimulated the expression of caspase-7.

Conclusion: The significant protective activity observed in this study indicated that EEBR might be beneficial in protecting kidney cells against cisplatin.


Implication for health policy/practice/research/medical education:

The results of this study can be used as a reference for the scientification and development of B. rotunda rhizomes as a candidate for nephroprotective phytopharmaceuticals, particularly for patients with cisplatin chemotherapy.

Please cite this paper as: Sujana D, Saptarini NY, Sumiwi SA, Levita J. The protective effect of Boesenbergia rotunda extract on cisplatin-exposed human embryonic kidney-293 cells by inhibiting the expression of kidney injury molecule-1, neutrophil gelatinase associated-lipocalin, NF-κB, and caspases. J Herbmed Pharmacol. 2023;12(1):147-152. doi: 10.34172/jhp.2023.14.

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Submitted: 18 Oct 2022
Revision: 30 Nov 2022
Accepted: 03 Dec 2022
ePublished: 31 Dec 2022
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