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J Herbmed Pharmacol. 2023;12(1): 168-175.
doi: 10.34172/jhp.2023.17

Scopus ID: 85147765682
  Abstract View: 805
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Original Article

In vivo and in vitro evaluation of antioxidant, antihemolytic, acute toxicity effects and high-performance liquid chromatography analysis of an aqueous extract of Moroccan Punica granatum L.

Ismail Elkoraichi 1 ORCID logo, Mounia Latif 1 ORCID logo, Othman El Faqer 1 ORCID logo, Rajaa Elaje 1,2 ORCID logo, Abderrahmane Thoume 3 ORCID logo, El Mostafa Mtairag 1 ORCID logo, Samira Rais 1,4* ORCID logo, Fouad Daoudi 1 ORCID logo

1 Immunology and Biodiversity Laboratory, Faculty of Sciences Ain Chock, Hassan II University, Casablanca, Morocco
2 Anatomopathology Unit 3, CUH Ibn Rochd Hospital, Casablanca, Morocco
3 Laboratory of Organic Synthesis, Extraction, and Valorization, Faculty of Sciences Aïn Chock, Hassan II University, Casablanca, Morocco
4 Department of Biology, Faculty of Science Ben M’Sick, Hassan II University, Casablanca, Morocco
*Corresponding Author: Samira Rais, Email: samirarais@hotmail.com

Abstract

Introduction: The use of pomegranates in Moroccan pharmacopeia is due to their healing and nutritional properties because of their richness in secondary molecules. The following study analyses the composition of the aqueous extract of Punica granatum peel and evaluates in vivo and in vitro antioxidant effects, hemolytic protection, and acute toxicity.

Methods: Quantification of the plant extract was realized by high-performance liquid chromatography (HPLC). The hemolytic assay was used for erythrocyte protection, while the in vitro antioxidant effect was evaluated by 2, 20-azinobis-(3-ethylbenzothiazoneline-6-sulphonic acid) (ABTS) and reducing ferric power (RFP) assays. The in-vivo antioxidant activity was tested by measuring levels of lipid peroxidation (LPO) in serum. The toxicological study was tested by oral administration of the extract to four groups of mice for 21 days, followed by a histopathological examination of the spleen.

Results: HPLC analysis showed the presence of some phenolic compounds such as coumarin, caffeic, gallic and syringic acids. The IC50 of the antioxidant assays were 254.49 ± 62.17 μg/mL and 40.265 ± 2.9 μg/mL for ABTS and reducing power, respectively. Furthermore, the thiobarbituric acid reactive substances (TBARS) assay showed the lowest levels at 150 mg/mL concentration. All of the concentrations used for hemolytic protection did not exceed 15% of hemolysis. Moreover, the toxicity test showed no sign of mortality, signs of weakness, or weight loss; also the histopathological examination of the spleen tissues showed the absence of any damage.

Conclusion: The peel extract of P. granatum showed good potential and could be exploited as a natural antioxidant and antihemolytic remedy, leading to the development of new drugs.


Implication for health policy/practice/research/medical education:

The aqueous extract of Moroccan pomegranate peel showed valuable in vivo and in vitro antioxidant efficiency and a protective effect on erythrocytes hemolysis. More assays are requested to support the obtained effects. Biocompounds present in the aqueous extract of pomegranate might be used as a substitute for drugs to cure antioxidant trouble.

Please cite this paper as: Elkoraichi I, Latif M, El Faqer O, Elaje R, Thoume A, Mtairag EM, et al. In vivo and in vitro evaluation of antioxidant, antihemolytic, acute toxicity effects and high-performance liquid chromatography analysis of an aqueous extract of Moroccan Punica granatum L. J Herbmed Pharmacol. 2023;12(1):168-175. doi: 10.34172/jhp.2023.17.

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Submitted: 19 Sep 2022
Revision: 25 Nov 2022
Accepted: 25 Nov 2022
ePublished: 31 Dec 2022
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