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J Herbmed Pharmacol. 2023;12(4): 592-596.
doi: 10.34172/jhp.2023.44897

Scopus ID: 85173780630
  Abstract View: 761
  PDF Download: 304

Short Communication

Antiparasitic and cytotoxicity effects of 7-hydroxy-4′- methoxy isoflavone against Leishmania major

Mahdi Aghaei 1* ORCID logo, Farhood Alizadegan 1 ORCID logo, Yosra Raziani 2 ORCID logo, Githa Kishore 1 ORCID logo, Massumeh Saadatmand 3 ORCID logo, Suja Ajoy Kumar 1 ORCID logo

1 Visveswarapura Institute of Pharmaceutical Sciences, Rajiv Gandhi University of Health Sciences, Bangalore, India
2 Nursing Department, Al-Mustaqbal University College, 51001 Hillah, Babylon, Iraq
3 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
*Corresponding Author: Mahdi Aghaei, Email: agham7271@gmail.com

Abstract

Introduction: Leishmaniasis caused by Leishmania spp. is observed in most parts of the world. Although, glucantime, a pentavalent antimony compound, and other synthetic drugs are broadly applied for leishmaniasis therapy; however, the use of these synthetic agents has some limitations. Hence, this study was designed to assess the inhibiting effects of 7-hydroxy4′-methoxyisoflavone (7HMI) against promastigote and amastigote stages of Leishmania major in vitro.

Methods: The MTT assay was applied to study the antileishmanial activity of 7HMI against promastigotes and its cytotoxicity effects on macrophage cells. The nitric oxide (NO) produced by the treated macrophage cells with 7HMI was also assessed.

Results: 7HMI considerably (P<0.05) inhibited the growth rate of promastigotes and amastigotes stages. The 50% inhibitory concentrations of 7HMI and glucantim were 11.3 and 15.4 µg/mL for promastigote and amastigote, respectively. 7HMI, especially at 1/3 IC50 and 1/2 IC50 concentrations, considerably triggered the NO release.

Conclusion: The current research findings reported the favorable antileishmanial effects of 7HMI against L. major with possible mechanisms such as reducing the infectivity rate of macrophage cells and provoking NO creation. Nevertheless, more research must be performed to clear its efficacy in animal model and then in human.


Implication for health policy/practice/research/medical education:

We reported the favorable antileishmanial effects of 7HMI against L. major with possible mechanisms such as reducing the infectivity rate of macrophage cells and provoking NO creation. Hence, 7HMI might be considered for preparation of a new drug against L. major Nevertheless, more researches must be performed to determine its efficacy in vivo and clinical subjects.

Please cite this paper as: Aghaei M, Alizadegan F, Raziani Y. Kishore G, Saadatmand M, Kumar SA. Antiparasitic and cytotoxicity effects of 7-hydroxy-4′-methoxy isoflavone against Leishmania major. J Herbmed Pharmacol. 2023;12(4):592-596. doi: 10.34172/jhp.2023.44897.

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Submitted: 17 Jan 2023
Revision: 17 Apr 2023
Accepted: 17 Apr 2023
ePublished: 10 Aug 2023
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