J Herbmed Pharmacol. 2023;12(3): 442-447.
doi: 10.34172/jhp.2023.49

Scopus ID: 85164958351
  Abstract View: 702
  PDF Download: 369

Original Article

Cognitive and histopathological effects of olive leaf extract in colchicine-induced hippocampal neurodegeneration in rats

Marwan Saad Azzubaidi 1* ORCID logo, Harmy Bin Mohamed Yusoff 1 ORCID logo, Imad Matloub Al-Ani 2 ORCID logo

1 Pharmacology Unit, Faculty of Medicine, Universiti Sultan Zainal Abidin, Kampus Kota, Kuala Terengganu, Malaysia
2 Department of Dentistry, Al-Hadi University College, Baghdad-10011, Iraq
*Corresponding Author: Marwan Saad Azzubaidi, Email: mazzubaidi@ gmail.com, , Email: mazzubaidi@unisza.edu.my


Introduction: Olive leaf extract (OLE) has robust anti-oxidant and anti-inflammatory properties. A toxic dose of colchicine (COL) injected into the hippocampus disrupts the microtubules’ neuronal structure causing it to be unstable and depolymerized. The objective of the current study was to evaluate the protective effects of OLE treatment on the CA1 hippocampal pyramidal cells of rats that are injected with intracranial COL.

Methods: Eighteen rats were divided into control, COL-injected, and OLE-treated-colchicine-injected (COL+OLE) groups (n=6). A vehicle solution was injected into the hippocampi of the control rats, whereas 15 µg/5 µL of COL was injected into the hippocampi of COL and COL+OLE groups. Forced oral treatment with 100 mg/kg OLE was commenced a week later and continued for 15 days. Short-term memory (STM) test using the Morris water maze (MWM) was performed followed by the retention probe memory test. Hippocampal samples from animals of all groups were collected for histopathological examination and qualitative assessment of the viable pyramidal cells at the CA1 hippocampal region.

Results: The control and COL+OLE groups demonstrated significantly better performance (P<0.05) in the STM test and its subsequent retention probe memory test as compared to the COL group. The morphology of the pyramidal cells of the COL+OLE treated rats was preserved, showing less distortion than the COL group.

Conclusion: OLE treatment led to a considerable preservation in the STM function of rats challenged with intrahippocampal COL injection. This memory improvement of the OLE might be attributed to its promising neuroprotective potential on hippocampal pyramidal cells.

Implication for health policy/practice/research/medical education:

OLE seems to have a considerable neuroprotective effect on hippocampal pyramidal cells improving memory improvement potential. More extensive evidence-based research is required to authenticate and screen its medicinal benefits and compare the biological activities of its active ingredients for the prevention and treatment of various diseases, including age-related neurodegeneration.

Please cite this paper as: Azzubaidi MS, Yusoff HM, Al-Ani IM. Cognitive and histopathological effects of olive leaf extract in colchicine-induced hippocampal neurodegeneration in rats. J Herbmed Pharmacol. 2023;12(3):442-447. doi: 10.34172/ jhp.2023.49.

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Submitted: 02 Feb 2023
Revision: 25 Apr 2023
Accepted: 29 Apr 2023
ePublished: 01 Jun 2023
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