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J Herbmed Pharmacol. 2024;13(2): 240-248.
doi: 10.34172/jhp.2024.48170

Scopus ID: 85192263751
  Abstract View: 438
  PDF Download: 200

Original Article

Exploring the anti-acne potential of Muntingia calabura L leaves against Staphylococcus epidermidis: In vitro and in silico perspective

Aloysius Imanadi Sambi 1 ORCID logo, Bakti Wahyu Saputra 1 ORCID logo, Agustina Setiawati 1* ORCID logo

1 Faculty of Pharmacy, Sanata Dharma University, Sleman, Yogyakarta 55284, Indonesia
*Corresponding Author: Agustina Setiawati, , Email: nina@usd.ac.id

Abstract

Introduction: Muntingia calabura is a medicinal plant possessing antimicrobial properties against various bacteria. The purpose of this study was to examine in vitro and in silico activity of the ethyl acetate fraction of M. calabura leaves against the acne-causing commensal bacterium, Staphylococcus epidermidis.

Methods: In this study, M. calabura leaves were extracted using ethanol and then further fractionated with ethyl acetate. The phytochemicals in the fraction were identified with thin layer chromatography (TLC). The activity of the fraction was then tested in S. epidermidis culture using the agar diffusion method. Additionally, the molecular docking of M. calabura phytochemicals constituents was simulated to teicoplanin-associated locus regulator (TcaR) of S. epidermidis.

Results: The ethyl acetate fraction of M. calabura exhibited robust antibacterial activity against S. epidermidis culture, resulting in inhibition zones ranging from 5 to 10 mm. The fraction was found to contain flavonoids, saponins, and tannins as identified constituents. Further, during the molecular docking analysis, stigmasterol and 7-methoxyflavone demonstrated binding to TcaR with a lower and comparable binding energy of -7.40 and -6.19 kcal/mol, respectively, compared to the control drug, Penicillin-G (-6.40 kcal/mol).

Conclusion: M. calabura has the potential to serve as a valuable source of active phytochemical compounds for addressing acne. Further studies are needed to isolate and evaluate each compound found in M. calabura individually against S. epidermidis.

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Submitted: 30 Jul 2023
Accepted: 12 Oct 2023
ePublished: 01 Apr 2024
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