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J Herbmed Pharmacol. 2024;13(2): 289-299.
doi: 10.34172/jhp.2024.49362

Scopus ID: 85190844402
  Abstract View: 318
  PDF Download: 227

Original Article

Multi-target mechanism of polyherbal extract to treat diabetic foot ulcer based on network pharmacology and molecular docking

Happy Elda Murdiana 1,2 ORCID logo, Retno Murwanti 3 ORCID logo, Nanang Fakhrudin 4,5 ORCID logo, Zullies Ikawati 3* ORCID logo

1 Doctorate Program of Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia
2 Departement of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Kristen Immanuel, Yogyakarta, Indonesia
3 Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia
4 Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia
5 Medicinal Plants and Natural Products Research Center, Faculty of Pharmacy, Universitas Gadjah Mada, Sekip Utara, Yogyakarta, Indonesia
*Corresponding Author: Zullies Ikawati, Email: zullies_ikawati@ugm.ac.id

Abstract

Introduction: Diabetic foot ulcer (DFU) potentially leads to loss of function, infections, hospitalization, lower-extremity amputation, and even death. The potential therapeutic efficacy of a polyherbal candidate named TIP-Heal was identified for treating DFU. TIP-Heal, which stands for Tinospora crispa, Isotoma longiflora, and Piper betle L var nigra, consists of extracts from these three herbs in a ratio of 2:1:1. The Indonesian population commonly uses these herbs due to their wound-healing properties. It is our interest to analyse the mechanism of the polyherbal extract using network pharmacology and molecular docking.

Methods: This study uses network pharmacology and molecular docking methods to analyze the multi-target mechanism of active compounds in TIP-Heal extract for DFU treatment. The proteins targeted by the bioactive chemical present in TIP-Heal and DFU were identified within a particular dataset with the keyword “homo sapiens.” The identified target proteins were assessed using gene ontology (GO) analysis, the Kyoto Encyclopaedia of Gene and Genomes (KEGG) pathways, protein-protein interactions (PPIs), and molecular docking.

Results: The critical proteins obtained were AKT serine/threonine kinase 1 (AKT1), caspase-3 (CASP3), epidermal growth factor receptor (EGFR), proto-oncogene tyrosine-protein kinase Src (SRC) and matrix metalloproteinase-9 (MMP-9). Several compounds, namely PubChem (Compound Identifier=CID: 5319898), 3-epiursolic acid, palmitic acid, and alpha-linolenic acid showed great potential as viable candidates to facilitate the healing process of DFU.

Conclusion: The findings of this study indicate that the TIP-Heal extract has the potential to be used as a natural herbal treatment for DFUs with the involvement of AKT1, CASP3, EFGR, and SRC proteins.


Implication for health policy/practice/research/medical education:

This study can provide preliminary information on the active ingredients in the polyherbal “TIP-Heal” and its potential molecular mechanisms for treating diabetic foot ulcers.

Please cite this paper as: Murdiana HE, Ikawati Z, Murwanti R, Fakhrudin N. Multi-target mechanism of polyherbal extract to treat diabetic foot ulcer based on network pharmacology and molecular docking. J Herbmed Pharmacol. 2024;13(2):289-299. doi: 10.34172/jhp.2024.49362

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Submitted: 14 Nov 2023
Accepted: 29 Feb 2024
ePublished: 01 Apr 2024
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