J Herbmed Pharmacol. 2022;11(4): 529-539.
doi: 10.34172/jhp.2022.61

Scopus ID: 85143274814
  Abstract View: 1298
  PDF Download: 741

Original Article

Phytochemical profile, anti-glycation effect, and advanced glycation end-products protein cross-link breaking ability of Sclerocarya birrea stem-bark crude extracts

Oluwaseyefunmi Iyabo Adeniran 1* ORCID logo, Andrew Munyalo Musyoki 2 ORCID logo, Lesibana Samuel Sethoga 3 ORCID logo, Motetelo Alfred Mogale 1 ORCID logo, Sechene Stanley Gololo 1 ORCID logo, Leshweni Jeremia Shai 4 ORCID logo

1 Department of Biochemistry, Sefako Makgatho Health Sciences University, Ga-Rankuwa, Pretoria, Republic of South Africa
2 Department of Microbiological Pathology, Sefako Makgatho Health Sciences University, Ga-Rankuwa, Pretoria, Republic of South Africa
3 Department of Chemistry, Sefako Makgatho Health Sciences University, Ga-Rankuwa, Pretoria, Republic of South Africa
4 Biomedical Sciences Department, Tshwane University of Technology, Arcadia campus, Pretoria, Republic of South Africa
*Corresponding Author: Email: seyefunmiadeniran@gmail.com


Introduction: Sclerocarya birrea stem-bark is widely used for the treatment of many medical conditions. Advanced glycation end-products (AGEs) are implicated in the pathogenesis of vascular complications of diabetes mellitus. The study, other than phytochemical composition, evaluated the anti-glycation and AGEs-protein cross-link breaking effects of S. birrea stem-bark extracts. Methods: Different S. birrea extracts and aminoguanidine (used as control) were incubated with bovine serum albumin (BSA) and glucose/fructose at 37oC for 40 days. Amounts of fluorescent AGEs (FAGEs) and immunogenic AGEs formed were determined. Anti-glycation activity percentage of each extract and aminoguanidine was calculated. Their AGEs-protein cross-link breaking abilities were also assessed. Standard techniques were employed for phytochemical screening. Volatile compounds were identified by means of gas chromatography mass spectrometry (GC-MS). Results: S. birrea stem-bark n-hexane extract was statistically more effective than aminoguanidine against the formation of total immunogenic AGEs (P<0.05). For FAGEs, ethyl acetate, methanol, and water extracts exerted significantly higher anti-glycation effects than aminoguanidine (P<0.001). Methanol extract exhibited the highest anti-glycation effect with an average IC50 value of 0.142 mg/mL against FAGEs. All extracts were effective in releasing BSA from the preformed collagen-AGEs-BSA cross-links. GC-MS enabled the identification of many biologically important compounds, including campesterol, stigmasterol, and 1-heptatricontanol. Conclusion: S. birrea stem-bark has a potential for usage in the management of complications in uncontrolled glucose metabolism.

Implication for health policy/practice/research/medical education: The results of this study may lead to the isolation of bioactive phytochemicals that might be used for the prevention of vascular complications of diabetes and other age-related or neurodegenerative diseases.
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Submitted: 27 Apr 2022
Revision: 30 Jun 2022
Accepted: 01 Jul 2022
ePublished: 21 Sep 2022
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